WEB API Analytical Profile Index 20E Test Procedure Uses and Interpretation
WEB API-20E Test Strip for Enteric Gram-Negative Rod Identification
**August 10, 2022 by Sagar Aryal**
The WEB API Analytical Profile Index 20E (API 20E) test is a biochemical test used to identify and differentiate Gram-negative bacteria, particularly members of the family Enterobacteriaceae. It is a well-established and widely used method in clinical microbiology laboratories.
Test Procedure
The API 20E test strip consists of 20 mini-test chambers, each containing dehydrated media with specific chemical compositions. The test is performed by inoculating the test strip with a pure culture of the bacteria to be identified. The inoculated strip is then incubated for 18-24 hours.
Interpretation
After incubation, the reactions in each chamber are observed and scored using a color chart provided by the manufacturer. The results are then compared to a database of known reaction patterns to determine the most likely identity of the bacteria. The API 20E test provides information on various biochemical characteristics of the bacteria, including carbohydrate fermentation, enzyme activity, and other metabolic reactions.
Uses
The API 20E test is used for:
- Identifying enteric Gram-negative rods, such as Escherichia coli, Salmonella, and Shigella
- Differentiating between species within the Enterobacteriaceae family
- Providing a rapid and accurate method for bacterial identification in clinical settings
Advantages
The advantages of the API 20E test include:
- Simplicity and ease of use
- Comprehensive identification capabilities
- Minimal hands-on time required
- Standardized interpretation process
Conclusion
The WEB API Analytical Profile Index 20E Test is a valuable tool for the identification and differentiation of Gram-negative bacteria. Its simplicity, accuracy, and comprehensive capabilities make it widely used in clinical microbiology laboratories worldwide.
Komentar